Pigment structure in the light-harvesting protein of the siphonous green alga Codium fragile - Laboratoire brestois de mécanique et des systèmes Accéder directement au contenu
Article Dans Une Revue Biochimica Et Biophysica Acta. Bioenergetics Année : 2021

Pigment structure in the light-harvesting protein of the siphonous green alga Codium fragile

Résumé

The siphonaxanthin-siphonein-chlorophyll-a/b-binding protein (SCP), a trimeric light-harvesting complex isolated from photosystem II of the siphonous green alga Codium fragile, binds the carotenoid siphonaxanthin (Sx) and/or its ester siphonein in place of lutein, in addition to chlorophylls a/b and neoxanthin. SCP exhibits a higher content of chlorophyll b (Chl-b) than its counterpart in green plants, light-harvesting complex II (LHCII), increasing the relative absorption of blue-green light for photosynthesis. Using low temperature absorption and resonance Raman spectroscopies, we reveal the presence of two non-equivalent Sx molecules in SCP, and assign their absorption peaks at 501 and 535 nm. The red-absorbing Sx population exhibits a significant distortion that is reminiscent of lutein 2 in trimeric LHCII. Unexpected enhancement of the Raman modes of Chls-b in SCP allows an unequivocal description of seven to nine non-equivalent Chls-b, and six distinct Chl-a populations in this protein.
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hal-03141625 , version 1 (13-02-2023)

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Paternité - Pas d'utilisation commerciale

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Simona Streckaité, Manuel J. Llansola-Portoles, Andrew A. Pascal, Cristian Ilioaia, Andrew Gall, et al.. Pigment structure in the light-harvesting protein of the siphonous green alga Codium fragile. Biochimica Et Biophysica Acta. Bioenergetics, 2021, 1862 (5), pp.148384. ⟨10.1016/j.bbabio.2021.148384⟩. ⟨hal-03141625⟩
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